Fidelity of chromosome segregation during mitosis is dependent on proper attachment of the microtubule to the centromere through the kinetochore. The centromere is a specialized region on the chromosome that specifies the location for building a kinetochore. Proper kinetochore-microtubule attachments are important for withstanding tension during metaphase while sister chromatids are joined and for their movement toward the poles during anaphase. Recent studies from our lab show that the centromeric histone variant Cse4 increases in copy number at the centromere during anaphase. We are using advanced microscopy techniques to explore the mechanism for centromere and kinetochore dynamics.