Core Centers


Research support services

An important cornerstone of the Stowers Institute’s philosophy is to provide a first-rate scientific infrastructure and easy access to the latest technology. Each core is headed by an expert in the field committed to the development, implementation and optimization of cutting-edge methodologies and instrumentation for the benefit of research programs at the institute. The small size of the institute’s research community allows the more than 100 highly trained scientists working in the research support cores to collaborate closely with Stowers investigators and to quickly adjust technical applications and support services to the requirements of individual labs. This not only promotes interdisciplinary research but also enables investigators to take advantage of the readily available expertise to expand their own research into new areas. What would take months and years elsewhere is often accomplished within days and weeks at the Stowers Institute.

 


 

Computational Biology

The Computational Biology facility provides service, training, collaborations and the development of new applications in the areas of sequence analysis, expression/ChIP analysis, general statistical and numerical analysis and support for scientific software and databases.

 

Cytometry

The Cytometry Center is a state-of-the-art flow cytometry laboratory that specializes in the identification and isolation of rare target cell populations and the use of cytometric tools for studies of developmental biology.

 

Electron Microscopy

The Electron Microscopy Facility assists with sample fixation and preparation for routine histology and ultra-structural analysis.

 

Histology

The Histology Facility offers expert service for the detection of gene and protein expression in tissue samples and performs histochemical staining. 

 

Imaging

The Imaging Facility specializes in the development and application of innovative imaging tools including tools such multicolor cell labeling and multispectral imaging strategies, photo-activatable fluorescent reporters and calcium indicators, deep tissue embryo imaging as well as laser capture microdissection and gene expression analysis.

 

Laboratory Animal Services

The Laboratory Animal Services (LAS) Facility provides the institute’s research staff with the highest quality laboratory animal care and support services using the most sophisticated equipment, advanced technology, and a progressive staff in a premier animal facility.

 

Media Preparation & Tissue Culture

The Media Preparation Facility prepares commonly used solutions and media, while the tissue culture laboratory grows widely used cell lines and can maintain, expand and store specific cell lines as requested.

 

Microscopy

The Microscopy Facility focuses on light as tool for imaging and measuring biological systems. It uses and develops software for image processing and data analysis.

 

Molecular Biology

The Molecular Biology Facility provides DNA sequencing, next-generation sequencing, microarrays, custom automation, site-directed mutagenesis, recombination-mediated genetic engineering, plasmid preps and assistance with the implementation of real time quantitative PCR.

 

Proteomics

The Proteomics Center implements a chromatography-based proteomic approach known as multidimensional protein identification technology (MudPIT) combined to spectral counting for label free proteomics quantitation. We routinely analyze affinity-purified protein complexes or subcellular fractions, while more in-depth analyses can be undertaken to find post-translational modifications in proteins of interest or to derive large scale protein interaction networks.

 

Reptile & Aquatics

The Reptile & Aquatics Facility provides the institute’s research staff with the highest quality laboratory animal care and husbandry services for non-mammalian species. It currently works with snakes, lizards, zebrafish, planaria and sea anemones.

 

Screening

The Screening Facility provides cutting edge technologies and robotics to perform automated high-throughput screening of genomic and compound libraries.  These platforms can be assayed using a multitude of endpoints including high-content imaging, fluorescence, time-resolve fluorescence, luminescence, absorbance and alphascreen.