Media Preparation & Tissue Culture


Media Preparation

The Media Preparation Facility serves to reduce the time and labor researchers must spend preparing reagents and growth media necessary for their various projects. Commonly used reagents such as Tris buffers and Luria broth are kept in stock to be used as needed by institute researchers. The facility prepares media used in larger volumes, such as for tissue culture and a variety of media plates poured to order.

Technology

The Media Preparation Facility uses the following equipment in its work:

  • Automated plate pourer for large plate orders
  • Level II Biosafety cabinet for tissue culture media preparation


Tissue culture

The Tissue Culture Core Laboratory comprises a group of experienced cell culture specialists who are dedicated to support the research staff in their tissue culture needs.  The Facility provides mycoplasma testing, cell banking, transfection services (stable cell line production) and large-scale cell expansion for Stowers researchers.  Cell lines include insect, mammalian, and embryonic stem cells.  Cells can be provided in single dishes or grown to large-scale in roller bottles, shaker flasks, or spinner flasks according to research needs.  Personal cell lines can be deposited to the core with the proper testing and IBC approval.  Training for new students or post-docs is available upon request.  

Technology

The Tissue Culture Core Laboratory uses the following equipment in its work:

  • Level II Biosafety cabinet (6)
  • Beckman Coulter Vi-Cell XR
  • Sanyo MCO-20A/C incubators (8)
  • Bellco Roller Bottle incubators (3)
  • Leica DMIL microscopes (2)
  • Lecia DMIL LED microscope
  • Eppendorf Multiporator
  • Beckman Allegra 6R centrifuge
  • Beckman Allegra X-15R centrifuge
  • Labnet Multigene thermocycler
  • Innova 2100 and 2300 shaking platforms (8)
  • Bellco Magnetic Stirrers (16)
  • Powers Scientific refrigerated incubator
  • Precision refrigerated incubators (2)

 

For more information, please contact:

Tari Parmely
Head, Media Preparation and Tissue Culture
816.926.4512
tjp@stowers.org