Microscopy


The Stowers Microscopy Center strives to enable every scientific member of the Stowers Institute to get the best light microscope images technically possible. Its staff of physicists, software specialists, chemists, and biologists trains users on the state-of-the-art equipment and assists with the set up optical experiments, the selection of the best microscope equipment for a given task and conducts microscopy experiments. In addition to supporting several image processing software packages, specialists are available to program custom solutions. The center also actively explores and develops new technologies.

Technology:

Standard microscopy:

  • Several wide-field microscopes with sensitive cameras and data-acquisition software
  • Several confocal microscopes with spectral detection capability
  • A fast line-scanning confocal for live imaging, fluorescence recovery after photo-bleaching (FRAP) and photo-activation experiments
  • Several two-photon microscopes with non-descanned detection

 

Special methods:

  • Structured illumination for 3D imaging
  • Total internal reflection microscopy (TIRF)
  • Fluorescence life-time imaging (FLIM)
  • Second-harmonics generation (SHG)
  • Fluorescence correlation spectroscopy (FCS) and related methods
  • Image correlation methods
  • Technology for high-content screening
  • Optical spectrometer
  • Photoactivated localization microscopy (PALM) with an optical resolution well below 100nm
  • Laser microdissection

 

Software:

  • Commercial and open source image processing and quantification packages
  • Software for 3D rendering and quantification
  • Deconvolution software
  • Cluster based image processing
  • Several programming environments to develop custom software solutions

 

For more information please visit the webpage of the Stowers Microscopy Center or contact:

Winfried Wiegraebe
Head of Microscopy
816.926.4415
wiw@stowers.org