Leanne Wiedemann

Leanne Wiedemann
Staff Scientist
Research Degree Coordinator, Open University

Professor, Department of Pathology and Laboratory Medicine,
   The University of Kansas School of Medicine



Research Summary

The simplest explanation of the focus of our research is to understand the how cells know where they are in the body and what they should become. The more complex description is to understand the regulatory basis of tissue patterning and morphogenesis during development, disease, and evolution. We explore gene networks using diverse genetic, genomic, biochemical, and imaging approaches. We also exploit the extensive conservation in hindbrain and somite segmentation between vertebrates by utilizing a variety of other model systems (human cells, chick, zebrafish, and lamprey) to investigate similarities and differences in patterning processes during vertebrate evolution.

Selected Publications

Smith, J.J., Timoshevskaya, N., Ye, C., Holt, C., Keinath, M.C., Parker, H.J., Cook, M.E., Hess, J.E., Narum, S.R., Lamanna, F., Kaessmann, H., Timoshevskiy, V.A., Waterbury, C.K.M., Saraceno, C., Wiedemann, L.M., Robb, S.M.C., Baker, C., Eichler, E.E., Hockman, D., Sauka-Spengler, T., Yandell, M., Krumlauf, R., Elgar, G., and Amemiya, C.T. The sea lamprey germline genome provides insights into programmed genome rearrangement and vertebrate evolution. Nat Genet.2018;50: 270-277. Abstract

McEllin JA, Alexander TB, Tumpel S, Wiedemann LM, Krumlauf R. Analyses of fugu hoxa2 genes provide evidence for subfunctionalization of neural crest cell and rhombomere cis-regulatory modules during vertebrate evolution. Dev Biol.2016;409:530-542. Abstract

Smith JJ, Kuraku S, Holt C, Sauka-Spengler T, Jiang N, Campbell MS, Yandell MD, Manousaki T, Meyer A, Bloom OE, Morgan JR, Buxbaum JD, Sachidanandam R, Sims C, Garruss AS, Cook M, Krumlauf R, Wiedemann LM, Sower SA, Decatur WA, Hall JA, Amemiya CT, Saha NR, Buckley KM, Rast JP, Das S, Hirano M, McCurley N, Guo P, Rohner N, Tabin CJ, Piccinelli P, Elgar G, Ruffier M, Aken BL, Searle SM, Muffato M, Pignatelli M, Herrero J, Jones M, Brown CT, Chung-Davidson YW, Nanlohy KG, Libants SV, Yeh CY, McCauley DW, Langeland JA, Pancer Z, Fritzsch B, de Jong PJ, Zhu B, Fulton LL, Theising B, Flicek P, Bronner ME, Warren WC, Clifton SW, Wilson RK, Li W. Sequencing of the sea lamprey (Petromyzon marinus) genome provides insights into vertebrate evolution. Nat Genet.2013;45:415-421, 421e411-412. Abstract

Wang P, Lin C, Smith ER, Guo H, Sanderson BW, Wu M, Gogol M, Alexander T, Seidel C, Wiedemann LM, Ge K, Krumlauf R, Shilatifard A. (2009). Global analysis of H3K4 methylation defines MLL family member targets and points to a role for MLL1-mediated H3K4 methylation in the regulation of transcriptional initiation by RNA polymerase II. Molecular and cellular biology29, 6074-6085. Abstract

Tümpel S, Wiedemann LM, Krumlauf R. (2009). Hox genes and segmentation of the vertebrate hindbrain. In HOX Genes, O. Pourquié, ed. (Burlington, Academic Press), pp. 103-137. Abstract

Tümpel S, Cambronero F, Sims C, Krumlauf R, Wiedemann LM. (2008) A regulatory module embedded in the coding region of Hoxa2 controls expression in rhombomere 2. Proc. Natl. Acad. Sci., U S A: 105, 20077-20082. Abstract

Tümpel S, Cambronero F, Ferretti E, Blasi F, Wiedemann LM, Krumlauf R. (2007) Expression of Hoxa2 in rhombomere 4 is regulated by a conserved cross-regulatory mechanism dependent upon Hoxb1. Dev. Biol. 302: 646-60. Abstract

He XC, Yin T, Grindley JC, Tian Q, Sato T, Tao WA, Dirisina R, Porter-Westpfahl KS, Hembree M, Johnson T, Wiedemann LM, Barrett TA, Hood L, Wu H, Li L. (2007) PTEN-deficient intestinal stem cells initiate intestinal polyposis. Nat. Genet. 39: 189-98. Abstract

Zhang J, He XC, Tong WG, Johnson T, Wiedemann LM, Mishina Y, Feng JQ, Li L. (2006) BMP signaling inhibits hair follicle anagen induction by restricting epithelial stem/progenitor cell activation and expansion. Stem Cells 25: 2826-2839. Abstract

Zhang J, Grindley JC, Yin T, Jayasinghe S, He XC, Ross JT, Haug JS, Rupp D, Porter-Westpfahl KS, Wiedemann LM, Wu H, Li L (2006) PTEN maintains haematopoietic stem cells and acts in lineage choice and leukaemia prevention. Nature 441, 518-522. Abstract

Tümpel S, Cambronero F, Wiedemann LM, Krumlauf R. (2006) Evolution of cis elements in the differential expression of two Hoxa2 coparalogous genes in pufferfish (Takifugu rubripes). Proc. Natl. Acad. Sci., U S A 103, 5419-5424. Abstract

Ferretti E, Cambronero F, Tümpel S, Longobardi E, Wiedemann LM, Blasi F, Krumlauf R. (2005). Hoxb1enhancer and control of rhombomere 4 expression: Complex interplay between PREP1-PBX1-HOXB1 binding sites. Mol. Cell. Biol. 25, 8541-52. Abstract

Serpente P, Tümpel S, Ghyselinck NB, Niederreither K, Wiedemann LM, Dollé P, Chambon P, Krumlauf R, Gould AP. (2005). Direct crossregulation between retinoic acid receptor b and Hox genes during hindbrain segmentation. Development 132, 503-513. Abstract

He XC, Zhang J, Tong WG, Tawfik O, Ross J, Scoville DH, Tian Q, Zeng X, He X, Wiedemann LM, Mishina Y, Li L. (2004). BMP signaling inhibits intestinal stem cell self-renewal through suppression of Wnt–b-catenin signaling. Nat Gen 36, 1117-1121. Abstract

Zhang J, Niu C, Ye L, Huang H, He X, Tong WG, Ross J, Haug J, Johnson T, Feng JQ, Harris S, Wiedemann LM, Mishina Y, Li L. (2003). Identification of the haematopoietic stem cell niche and control of the niche size. Nature 425, 836-841. Abstract