Histology


The Histology team serves Stowers researchers by implementing basic to molecular methods as well as developing new histotechnologies. The team’s focus is identifying cellular components of tissues and organs across a variety of organisms throughout development, regeneration, and disease states. Comprised of histotechnologists and scientists with backgrounds ranging from cytogenetics to neuroscience, the team provides customized collaborative research services, trains users on a variety of histotechniques, assists in selecting the best histological method for a given task, and conducts tissue processing, sectioning and staining.

Technology

Routine Methods:

  • Paraffin microtomy
  • Cryotomy
  • Vibratome sectioning
  • Plastic sectioning
  • H&E and special stains

 

Special Methods:

  • Antibody verification and validation
  • Automatic and manual slide-mount and whole mount in situ hybridization (ISH)
  • Analysis of mRNA and protein at single molecule level with single cell resolution
  • Multiplexing of immunohistochemistry and in situ hybridization assays
  • Expansion microscopy
  • Spatial interrogation of gene expression across tissues
  • Chromosome and synaptonemal complex spread (Meiosis oocyte and spermatocyte)
  • DNA fluorescence in situ hybridization (FISH)/immunohistochemistry multiplexing
  • Fiber FISH
  • Yeast cytology

 

For more information, please contact:

Yongfu Wang
Head, Histology
816.926.4510
yow@stowers.org