Screening

The Screening team assists Stowers scientists with experiments that test large numbers of molecules or conditions, including high-throughput chemical and genomic screens as well as high-throughput biological assays using advanced high-content imaging and robotic liquid handling platforms.

Research Services 

The Screening Facility is a state-of-the-art laboratory that performs high-throughput screening of genomic (cDNA and siRNA) and compound libraries. The Screening team assists researchers with the development of biologically relevant assays using current as well as novel technologies to perform primary, secondary, and counter-screens that identify molecules or proteins modulating their biological endpoint. Numerous assay endpoints are supported in small microtiter formats (96, 384, and 1536 wells) including: high-content imaging, fluorescence intensity, fluorescence polarization, time-resolve fluorescence, luminescence, alphascreen, and absorbance. In addition, the screening team assists researchers with high-throughput biology projects to miniaturize and automate bench-level techniques, in order to increase the number of samples and/or replicates analyzed, thereby increasing reproducibility and decreasing the cost per sample and assay time.

Technology 
  • PerkinElmer Janus G3 Automated Workstation with 96 and 384w heads and Varispan 8-channel pipetting arm with stackers
  • PerkinElmer Opera Phenix high content imager with environmental chamber integrated with a robotic arm to transfer plates from a Liconic 44 plate live cell incubator.
  • PerkinElmer Operetta high content imager with environmental chamber
  • Hudson Platecrane Ex integrated with a Hudson automated barcode printer, plate carousel and Alps 3000 heat sealer
  • PerkinElmer Envision multimodal plate readers
  • Tecan Spark plate reader with environmental chamber
  • Tecan Infinity plate reader
  • Biotek Multiflo liquid dispenser with peristaltic pump and syringe injection systems
  • Integra Viafill liquid dispenser for 96w, 384w and 1536w plates
  • Biotek EL405 plate washer and dispenser
Team Contact 
Juliana Conkright-Fincham, PhD
Head, Screening

Julie Conkright received her dual bachelor's degrees in biology and microbiology from Kansas State University and served as a Howard Hughes Medical Institute predoctoral intern during her senior year. At Cincinnati’s Children’s Medical Center, she studied surfactant biology and neonatal respiratory distress as part of her doctoral thesis and received her PhD in molecular and developmental biology from the University of Cincinnati College of Medicine. After completing a postdoctoral fellowship in Bill Balch’s laboratory at The Scripps Research Institute in La Jolla, CA, where she worked on the molecular mechanism of alpha-1-antitrypsin (a1AT) secretion, Conkright moved to Florida to manage the Cell-based Screening Facility at The Scripps Research Institute in Jupiter, FL. She aided investigators in genomic and compound screening as well as participated in small molecule probe development as a part of a center-based initiative in the NIH Molecular Libraries Program. In 2010, Conkright was recruited to the Stowers Institute to establish an advanced screening core that can handle a wide array of high content imaging and biochemical technologies in mammalian and non-mammalian systems.

Featured Publications 
Paul P, Ramachandran S, Xia, S, Unruh JR, Conkright-Fincham J, Li R. PLos One. 2019;14(5):e0216220.
Suganuma T, Swanson SK, Gogol M, Garrett TJ, Conkright-Fincham J, Florens L, Washburn MP, Workman JL. Cell Rep. 2018;24(6):1585-1596.
Mulla W, Seidel CW, Zhu J, Tsai HJ, Smith SE, Singh P, Bradford WD, McCroskey S, Nelliat AR, Conkright J, Peak A, Malanowski KE, Perera AG, Li R. ELife. 2017;6:e27991.
Banks CA, Boanca G, Lee ZT, Eubanks CG, Hatten GL, Peak A, Weems LE, Conkright JJ, Florens L, Washburn MP. Mol Cell Proteomics. 2016;15(11):3435-3449.
Chen G, Mulla W, Kucharavy A, Tsai HJ, Rubinstein B, Conkright J, McCroskey S, Bradford W, Weems L, Haug JS, Seidel CW, Berman J, Li R. Cell. 2015;160(4):771-784.
Sela D, Conkright JJ, Chen L, Gilmore J, Washburn MP, Florens L, Conaway RC, Conaway JW. J Biol Chem. 2013;288(36):26179-87.
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