The Transgenic and Reproductive Technologies group provides specialized services for Stowers scientist that utilize genetically modified organisms.
The Transgenic and Reproductive Technologies (TRT) group is composed of team members who possess extensive knowledge and expertise to create genetically modified organisms, using advanced technology. This group works in partnership with researchers from investigator labs and other Technology Centers to develop and protect the variants that are used in ongoing scientific studies.
The TRT group offers a variety of technical services including applying CRISPR/Cas9 and standard transgenic techniques to well-studied and new research organisms, rederivation of existing health-compromised models, the recovery and rescue of strains through in vitro fertilization and the cryopreservation and storage of germplasm from the research organisms.
- NEPA21 Electro-Kinetic Transfection System
- WPI MICRO-ePORE™ pinpoint cell penetrator
- BLS #CF-150 Electrofusion apparatus
- FTS Systems BC-III-80 Bio-Cool Controlled Rate Freezer
- Hamilton Thorne XYClone laser ®
- Leica Biosystems Angle Two Small Animal Stereotaxic Instrument
- Eppendorf FemtoJetR 4i electronic microinjector
- Nikon ECLIPSE Ti2U inverted microscope with Differential Interference Contrast (Nomarski) Optics
- Leica DMI3000B Inverted Microscope with brightfield, darkfield, phase, modulation, and differential interference optics capability.
- Nikon Eclipse TE2000U with Hoffman Modulation Contrast Optics and equipped with fluorescence capability.