Transgenic & Reproductive Technologies

The Transgenic and Reproductive Technologies group provides specialized services for Stowers scientist that utilize genetically modified organisms.

Research Services 

The Transgenic and Reproductive Technologies (TRT) group is composed of team members who possess extensive knowledge and expertise to create genetically modified organisms, using advanced technology. This group works in partnership with researchers from investigator labs and other Technology Centers to develop and protect the variants that are used in ongoing scientific studies.

The TRT group offers a variety of technical services including applying CRISPR/Cas9 and standard transgenic techniques to well-studied and new research organisms, rederivation of existing health-compromised models, the recovery and rescue of strains through in vitro fertilization and the cryopreservation and storage of germplasm from the research organisms.

Technology 
  • NEPA21 Electro-Kinetic Transfection System
  • WPI MICRO-ePORE™ pinpoint cell penetrator
  • BLS #CF-150 Electrofusion apparatus
  • FTS Systems BC-III-80 Bio-Cool Controlled Rate Freezer
  • Hamilton Thorne XYClone laser ®
  • Leica Biosystems Angle Two Small Animal Stereotaxic Instrument
  • Eppendorf FemtoJetR 4i electronic microinjector
  • Nikon ECLIPSE Ti2U inverted microscope with Differential Interference Contrast (Nomarski) Optics
  • Leica DMI3000B Inverted Microscope with brightfield, darkfield, phase, modulation, and differential interference optics capability.
  • Nikon Eclipse TE2000U with Hoffman Modulation Contrast Optics and equipped with fluorescence capability.
Team Contact 
Featured Publications 
Pang J, Le L, Zhou Y, Tu R, Hou Q, Tsuchiya D, Thomas N, Wang Y, Yu Z, Alexander R, Thexton M, Lewis B, Corbin T, Durnin M, Li H, Ashery-Padan R, Yan D, Xie T. Cell Rep. 2021;34:108603. doi: 108610.101016/j.celrep.102020.108603.
Kushawah G, Hernandez-Huertas L, Abugattas-Nunez Del Prado J, Martinez-Morales JR, DeVore ML, Hassan H, Moreno-Sanchez I, Tomas-Gallardo L, Diaz-Moscoso A, Monges DE, Guelfo JR, Theune WC, Brannan EO, Wang W, Corbin TJ, Moran AM, Sanchez Alvarado A, Malaga-Trillo E, Takacs CM, Bazzini AA, Moreno-Mateos MA.  Dev Cell. 2020;54:805-817 e807.
Aoto K, Sandell LL, Butler Tjaden NE, Yuen KC, Watt KE, Black BL, Durnin M, Trainor PA. Dev Biol. 2015 Jun 1;402(1):3-16. doi: 10.1016/j.ydbio.2015.02.022. Epub 2015 Mar 17. PMID: 25794678
Suraneni P, Rubinstein B, Unruh JR, Durnin M, Hanein D, Li R. J Cell Biol. 2012;197:239-251.
He J, Ma L, Kim S, Schwartz J, Santilli M, Wood C, Durnin MH, Yu CR. Version 2. J Neurosci. 2010 Jun 2;30(22):7473-83. doi: 10.1523/JNEUROSCI.0825-10.2010. PMID: 20519522
Iulianella A, Sharma M, Durnin M, Vanden Heuvel GB, Trainor PA. Development. 2008 Feb;135(4):729-41. doi: 10.1242/dev.013276. PMID: 18223201
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