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Transgenic & Reproductive Technologies

The Transgenic and Reproductive Technologies group provides specialized services for Stowers scientists that utilize genetically modified organisms.

Overview of Services

Research Services

The Transgenic and Reproductive Technologies group is composed of team members who possess extensive knowledge and expertise to create genetically modified organisms using advanced technology. This group works in partnership with researchers from investigator labs and other Technology Centers to develop and protect the variants that are used in ongoing scientific studies.

The team offers a variety of technical services including applying CRISPR/Cas9 and standard transgenic techniques to well-studied and new research organisms, rederivation of existing health-compromised models, the recovery and rescue of strains through in vitro fertilization and the cryopreservation and storage of germplasm from the research organisms.


  • NEPA21 Electro-Kinetic Transfection System
  • WPI MICRO-ePORE™ pinpoint cell penetrator
  • BLS #CF-150 Electrofusion apparatus
  • FTS Systems BC-III-80 Bio-Cool Controlled Rate Freezer
  • Hamilton Thorne XYClone laser ®
  • Leica Biosystems Angle Two Small Animal Stereotaxic Instrument
  • Eppendorf FemtoJetR 4i electronic microinjector
  • Nikon ECLIPSE Ti2U inverted microscope with Differential Interference Contrast (Nomarski) Optics
  • Leica DMI3000B Inverted Microscope with brightfield, darkfield, phase, modulation, and differential interference optics capability.
  • Nikon Eclipse TE2000U with Hoffman Modulation Contrast Optics and equipped with fluorescence capability.
A microscope at the Stowers Institute

Genetically modified organisms

Cloning, surgery, preservation, in vitro fertilization are just some of the capabilities of the Transgenic and Reproductive Technologies team.

Featured Publications

NOTCH Signaling Controls Ciliary Body Morphogenesis and Secretion by Directly Regulating Nectin Protein Expression

Pang J, Le L, Zhou Y, Tu R, Hou Q, Tsuchiya D, Thomas N, Wang Y, Yu Z, Alexander R, Thexton M, Lewis B, Corbin T, Durnin M, Li H, Ashery-Padan R, Yan D, Xie T. Cell Rep. 2021;34:108603. doi: 108610.101016/j.celrep.102020.108603.

CRISPR-Cas13d Induces Efficient mRNA Knockdown in Animal Embryos

Kushawah G, Hernandez-Huertas L, Abugattas-Nunez Del Prado J, Martinez-Morales JR, DeVore ML, Hassan H, Moreno-Sanchez I, Tomas-Gallardo L, Diaz-Moscoso A, Monges DE, Guelfo JR, Theune WC, Brannan EO, Wang W, Corbin TJ, Moran AM, Sanchez Alvarado A, Malaga-Trillo E, Takacs CM, Bazzini AA, Moreno-Mateos MA.  Dev Cell. 2020;54:805-817 e807.

Mef2c-F10N enhancer driven beta-galactosidase (LacZ) and Cre recombinase mice facilitate analyses of gene function and lineage fate in neural crest cells.

Aoto K, Sandell LL, Butler Tjaden NE, Yuen KC, Watt KE, Black BL, Durnin M, Trainor PA. Dev Biol. 2015;402:3-16.

The Arp2/3 complex is required for lamellipodia extension and directional fibroblast cell migration.

Suraneni P, Rubinstein B, Unruh JR, Durnin M, Hanein D, Li R. J Cell Biol. 2012;197:239-251.

Distinct Signals Conveyed to Pheromone Concentrations to the Mouse Vomeronasal Organ.

He J, Ma L, Kim S, Schwartz J, Santilli M, Wood C, Durnin MH, Yu CR. J Neurosci. 2010;30:7473-7483.

Cux2 functions downstream of Notch signaling to regulate dorsal interneuron formation in the spinal cord.

Iulianella A, Sharma M, Vanden Heuvel GB, Trainor PA. Development. 2009;136:2329-2334.

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