“There was a split at the origin of vertebrates between jawless and jawed around 500 million years ago,” said Alice Bedois, Ph.D., a former predoctoral researcher in the Krumlauf Lab and lead author on the study. “We wanted to understand how the vertebrate brain evolved and if there was something unique to jawed vertebrates that was lacking in their jawless relatives.”

Previous work from the Krumlauf Lab and the lab of Marianne Bronner, Ph.D., at the California Institute of Technology had identified that the genes structuring and subdividing the sea lamprey hindbrain are identical to those in jawed vertebrates including humans.

However, these genes are part of an interconnected network or circuit that needs to be initiated and directed to build the hindbrain correctly. The new study identified a common molecular cue, while known to direct head-to-tail patterning in a wide variety of animals, as part of the gene circuitry guiding hindbrain patterning in sea lampreys.

“We found that not only are the same genes but also the same cue is involved in sea lamprey hindbrain development, suggesting this process is ancestral to all vertebrates,” said Bedois.

This cue is called retinoic acid, commonly known as vitamin A. While the researchers knew that retinoic acid cues the gene circuitry to build the hindbrain in complex species, it was not thought to be involved for more primitive animals like sea lampreys. Surprisingly, they found that the sea lamprey core hindbrain circuit is also initiated by retinoic acid, providing evidence that these sea monsters and humans are much more closely related than anticipated.

“People thought that because sea lampreys lack a jaw, their hindbrain was not formed like other vertebrates,” said Krumlauf. “We have shown that this basic part of the brain is built in exactly the same way as mice and even humans.”

There are well known signaling molecules that inform the fate of cells during development. Now, the researchers have found that retinoic acid is another major player that cues vital steps in development like formation of the brain stem. In addition, if hindbrain formation is a conserved feature for all vertebrates, other mechanisms must be responsible to explain their incredible diversity.

“We all derived from a common ancestor,” said Bedois. “Sea lampreys have provided an additional clue. Now we need to look even further back in evolutionary time to discover when the gene circuitry governing hindbrain formation first evolved.”

Additional author: Marianne Bronner, Ph.D.

This work was funded by the National Institute of Neurological Disorders and Stroke (award: R35NS111564) of the National Institutes of Health (NIH) and by institutional support from the Stowers Institute for Medical Research. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.

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