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Custom Protein Resources

The Custom Protein Resources team produces a variety of protein-based products that support research at the Institute.

Overview of Services

Research Services

Custom Protein Resources (CPR) is a collaborative research support service that enables researchers to isolate and develop protein-based reagents to propel their research. The CPR Team strives to produce high-quality reagents and collaborate closely with each researcher to validate those reagents in their specific experimental system. The CPR Team can design, express, and isolate a wide variety of proteins from prokaryotic and eukaryotic expression systems and screen for novel reagents such as nanobodies. The team provides protein assay design expertise and access to protein analysis instrumentation.


  • ÄKTA Start protein purification system (2)
  • ÄKTA Go protein purification system
  • Sartoris Octet R4 Biolayer Interferometry (BLI) Instrument
  • Malvern PEAQ ITC
  • Jasco J1500 Circular Dichroism Spectrophotometer
  • BioComp Gradient Station with Triax flow cell
  • Agilent 1200 series HPLC

Learn More

Special Methods

  • Protein engineering
  • Protein expression and purification
  • Protein-based assay design
  • Nanobody screening and validation
  • Kinetic analysis of protein interactions

Team Contact

William Bret Redwine

Head, Custom Protein Resources

Stowers Institute for Medical Research

Portrait of Bret Redwine

Resources and Technologies

Custom Protein Resources supports Stowers Institute scientists using a multi-disciplinary, molecular level approach to protein engineering and project-customized protein-based resources and reagents. In-depth consultation with scientists results in delivery of a physical product or an intellectual solution for a given research objective.


Fast Protein Liquid Chromatography (FPLC)

FPLC is used to isolate or characterize proteins of interest based on properties such as the presence of an epitope tag, size, ionic charge, or hydrophobic or hydrophilic nature, to name a few.

The Sartorious Octet® platform, which enables label-free biomolecular interaction analysis based on Bio-Layer Interferometry technology.

Sartorius Octet R4 BLI Instrument

Biolayer interferometry (BLI) is a versatile technique that can interrogate the kinetics of protein-protein interactions, determine the concentration of proteins in complex mixtures, and more. BLI biosensors utilize light to capture real-time measurements of the kinetics, or rate of interactions between proteins as well as their affinity to each other.

Featured Publications

A High-throughput Automated ELISA Assay for Detection of IgG Antibodies to the SARS-CoV-2 Spike Protein

Conkright-Fincham J, Tomomori-Sato C, McGhee R, Leslie EM, Beucher CJ, Weems LE, Sato S, Redwine WB, Weaver KJ, Miller BD, Delventhal KM, Kary JJ, Koebbe AB, Dean A, Witt JL, Remy LM, Parmely TJ, Zhao C, Wang Y, Conaway JW, Unruh JR. Bio-protocol. 2022;12:e4301. doi: 4310.21769/BioProtoc.24301.

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