The Genome Engineering team supports the CRISPR-Cas9 genome engineering technology in a number of model systems including mouse, zebrafish, cavefish, fruit fly, sea anemone, apple snail, and mammalian cultured cells. They provide services for guideRNA design and assembly, donor construct design and assembly, preparation of reagents for injection or electroporation, and engineering of mammalian cell lines. The team uses pooled amplicon sequencing for detection and analysis of mutations in samples. They also provide support for pooled library screens, such as CRISPRi or CRISPRa, through library amplification, validation, and preparation of samples treated in the screen. They are responsible for maintaining and distributing clone and vector library collections to researchers within the Institute.