Genome Engineering

The Genome Engineering team assists Stowers researchers in the precise and specific alteration of genetic material of research organisms and cultured cells.

Research Services 

The Genome Engineering team supports the CRISPR-Cas9 genome engineering technology in a number of model systems including mouse, zebrafish, cavefish, fruit fly, sea anemone, apple snail, and mammalian cultured cells. They provide services for guideRNA design and assembly, donor construct design and assembly, preparation of reagents for injection or electroporation, and engineering of mammalian cell lines. The team uses pooled amplicon sequencing for detection and analysis of mutations in samples. They also provide support for pooled library screens, such as CRISPRi or CRISPRa, through library amplification, validation, and preparation of samples treated in the screen. They are responsible for maintaining and distributing clone and vector library collections to researchers within the Institute.

Technology 
  • ViaFlo 96
  • MicroPro 300 Benchtop 96 Channel Pipettor
  • MiSeq System
Team Contact 
Kym Delventhal
Head, Genome Engineering

Kym Delventhal joined the Institute in 2003 and has served in numerous roles within Molecular Biology and now Genome Engineering. As Head of Genome Engineering, Delventhal manages a team that provides support to all labs at the Institute. Delventhal graduated from Kansas State University with degrees in microbiology and chemistry. While there, she was a research assistant in the Wheat Genetics Resource Center. Delventhal is a member of the Association of Biomolecular Resource Facilities (ABRF) and served as chair of the ABRF-Genome Editing Research Group from 2017 to 2019.

Featured Publications 
Ikmi A, McKinney SA, Delventhal KM, Gibson MC. Nat Comm. 2014;5:5486, doi10.1038/ncomms6486.
Delventhal K. Journal of Biomolecular Techniques: JBT 24(Suppl) May 2013
Friederichs JM, Ghosh S, Smoyer CJ, McCroskey S, Miller BD, Weaver KJ, Delventhal KM, Unruh J, Slaughter BD, Jaspersen SL. PLoS Genet. 2011;7:e1002365.
Cornier AS, Staehling-Hampton K, Delventhal KM, Saga Y, Caubet JF, Sasaki N, Ellard S, Young E, Ramirez N, Carlo SE, Torres J, Emans JB, Turnpenny PD, Pourquié O. Am J Hum Genet. 2008;82:1334-1341.
Nakanishi S, Sanderson BW, Delventhal KM, BradfordWD, Staehling-Hampton K, Shilatifard A. Nat Struct Mol Biol. 2008;15:881-888.
© 2021 Stowers Institute for Medical Research