The Genome Engineering team supports the CRISPR-Cas technology in several model systems, including mouse, zebrafish, cavefish, fruit fly, sea anemone, apple snail, and mammalian cell lines. Their services include guideRNA design and assembly, donor template design and assembly, reagent preparation for injection or electroporation, and engineering mammalian cell lines.

The team also provides a targeted deep sequencing service for detecting and identifying engineered mutations, and support for pooled screens, such as CRISPR a/i. Other services include building or acquiring new pooled libraries, followed by preparation and validation by next-generation sequencing. Resulting screened cell populations are processed for genomic DNA purification, target amplification, and next-generation sequencing. The team is responsible for maintaining and distributing 150,000+ clones and vectors. The thirty-four collections of clones include yeast, fly, mouse, human, and expression vectors.