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Histology

The Histology team provides histotechnical instrumentation, technical support, and collaborative services to Stowers Institute scientists whose research involves the microscopic anatomy of biological tissues and cells.

The Histology Center is hiring!

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Overview of Services

Research Services

Comprised of histotechnologists and biologists with backgrounds in cytogenetics, molecular biology and neuroscience, the Histology Team collaborates with Stowers colleagues on projects to understand the spatial composition of RNA, protein, or other components in various types of samples, ranging from chromosome spread, cells, and tissue sections to whole mount organisms. The team has earned a reputation for creativity in developing histological methods to enable scientific discoveries.

Technologies

The Stowers Histology center is a full-service histology laboratory that offers a variety of histological techniques to Institute members. We process fresh, paraffin-embedded and frozen tissue for routine histology, special stains, immunohistochemical assays, tissue clearing, in-situ hybridization, expansion microscopy, and spatial transcriptomics & proteomics. Additionally, we provide investigators with training, consultation and advice on how to best use histology services.

  • LifeCanvas SmartClear II Pro and SmartLabel
  • Intavis InsituPro Vsi robot
  • HTXImaging M3+ Matrix Sprayer
  • Thermo Cryostar NX70
  • Thermo Cytospin4
  • HybEZ-II hybridization oven
  • Biogenex EZ-Retriever
  • Milestone Pathos Delta processor
  • Leica Autostainer XL and CV5030
  • Leica EG1150 embedding center
  • Leica cryoJane ECU500
  • Bio-Rad C1000Touch Thermal cycler
  • Leica VT1200 and VT1000S vibratome
  • Leica HistoCore Autocut microtome
  • Thermo Microm HM355S microtome
  • Leica CM3050S cryostat

Team Contact

Yongfu Wang

Head, Histology

Stowers Institute for Medical Research

Portrait of Yongfu Wang

Featured Publications

Step-by-step preparation of mouse eye sections for routine histology, immunofluorescence, and RNA in situ hybridization multiplexing

Pang J, Thomas N, Tsuchiya D, Parmely T, Yan D, Xie T, Wang Y. STAR Protoc. 2021;2:100879. doi: 100810.101016/j.xpro.102021.100879..

Changes in regeneration-responsive enhancers shape regenerative capacities in vertebrates

Wang W, Hu CK, Zeng A, Alegre D, Hu D, Gotting K, Ortega Granillo A, Wang Y, Robb S, Schnittker R, Zhang S, Alegre D, Li H, Ross E, Zhang N, Brunet A, Sanchez Alvarado A. Science. 2020;369: eaaz3090. doi: 10.1126/science.aaz3090.

Adaptation to low parasite abundance affects immune investment and immunopathological responses of cavefish

Peuß R, Box AC, Chen S, Wang Y, Tsuchiya D, Persons JL, Kenzior A, Maldonado E, Krishnan J, Scharsack JP, Slaughter BD, Rohner N. Nat Ecol Evol. 2020;4:1416-1430.

Alkaline phosphatase-based chromogenic and fluorescence detection method for BaseScope™ In Situ hybridization

Wang Y, Xu W, Maddera L, Tsuchiya D, Thomas N, Yu CR, Parmely T.  J Histotechnol. 2019;42:193-201.

Combined expansion microscopy with structured illumination microscopy for analyzing protein complexes

Wang Y, Yu Z, Cahoon CK, Parmely T, Thomas N, Unruh JR, Slaughter BD, Hawley RS. Nat Protoc. 2018;13:1869-1895.

Prospectively Isolated Tetraspanin(+) Neoblasts Are Adult Pluripotent Stem Cells Underlying Planaria Regeneration

Zeng A, Li H, Guo L, Gao X, McKinney S, Wang Y, Yu Z, Park J, Semerad C, Ross E, Cheng LC, Davies E, Lei K, Wang W, Perera A, Hall K, Peak A, Box A, Sánchez Alvarado A. Cell. 2018;173:1593-1608.e20.

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