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Cytometry

The Cytometry team assists Stowers Institute scientists by providing technical services and collaborative support for the comprehensive measurement of cells and their properties.

Overview of Services

Research Services

The Cytometry facility is a state-of-the-art laboratory that provides Stowers Institute researchers with a resource for analytical and preparative studies of cells using flow cytometry. The Cytometry team provides a full range of services including routine sample processing, cell staining, data analysis and strategic planning for novel assays and custom cell sorting applications. Collaborative projects encompass a broad scope of research topics and often result from a group effort among several of the Institute’s scientific support teams.

Technologies

Cytometry Analyzers

  • BioRad ZE5 Cell Analyzer
  • Sony EC800 Flow Cytometry Analyzer

High Speed Cell Sorters

  • BD FACSymphony S6 Cell Sorter
  • BD Influx Cell Sorter
  • BD FACSMelody Cell Sorter

Imaging Equipment

  • Amnis ImageStream MkII Imaging Flow Cytometer
  • Nexcelom Celigo Imaging Cytometer

Macroparticle Sorter

  • Union Biometrica CoPas Large Particle Sorter

Learn More

Team Contact

Jeffery Haug

Head, Cytometry

Stowers Institute for Medical Research

Portrait of Jeff Haug

Services we offer

The Cytometry Facility at the Stowers Institute for Medical Research offers a wide variety of cell sorting and cytometry analysis services, data analysis pipelines, and custom reagent protein consultation and production.

Advanced cell sorting and analysis

Image cytometry

Automation and Data Analysis

Featured Publications

Identification of Stem Cells from Large Cell Populations with Topological Scoring

Sardiu ME, Box AC, Haug JS, Washburn MP. Mol Omics. 2021;17:59-65.

Evaluating the Effects of Cell Sorting on Gene Expression

Box A, DeLay M, Tighe S, Chittur SV, Bergeron A, Cochran M, Lopez P, Meyer EM, Saluk A, Thornton S, Brundage K. J Biomol Tech : JBT. 2020;31:100-111.

Adaptation to low parasite abundance affects immune investment and immunopathological responses of cavefish

Peuß R, Box AC, Chen S, Wang Y, Tsuchiya D, Persons JL, Kenzior A, Maldonado E, Krishnan J, Scharsack JP, Slaughter BD, Rohner N. Nat Ecol Evol. 2020;4:1416-1430.

Defining the expression of piRNA and transposable elements in Drosophila ovarian germline stem cells and somatic support cells.

Story B, Ma X, Ishihara K, Li H, Hall K, Peak A, Anoja P, Park J, Haug J, Blanchette M, Xie T.  Life Sci Alliance. 2019;2:e201800211. doi: 10.26508/lsa.201800211.

scRNA-Seq reveals distinct stem cell populations that drive hair cell regeneration after loss of Fgf and Notch signaling

Lush ME, Diaz DC, Koenecke N, Baek S, Boldt H, St Peter MK, Gaitan-Escudero T, Romero-Carvajal A, Busch-Nentwich EM, Perera AG, Hall KE, Peak A, Haug JS, Piotrowski T.  Elife. 2019;8:e44431. doi: 44410.47554/eLife.44431.

Suppression of m(6)A reader Ythdf2 promotes hematopoietic stem cell expansion

Li Z, Qian P, Shao W, Shi H, He XC, Gogol M, Yu Z, Wang Y, Qi M, Zhu Y, Perry JM, Zhang K, Tao F, Zhou K, Hu D, Han Y, Zhao C, Alexander R, Xu H, Chen S, Peak A, Hall K, Peterson M, Perera A, Haug JS, Parmely T, Li H, Shen B, Zeitlinger J, He C, Li L. Cell Res. 2018. Author Correction: Cell Res 2018:1-14. https://doi.org/10.1038/s41422-018-0072-0. Published online July 2018.;28:904-917.

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