Cytometry

The Cytometry team assists Stowers scientists by providing technical services and collaborative support for the comprehensive measurement of cells and their properties.

Research Services 

The Cytometry Facility is a state-of-the-art laboratory that provides Stowers researchers with a resource for analytical and preparative studies of cells using flow cytometry. The Cytometry team provides a full range of services including routine sample processing, cell staining, data analysis and strategic planning for novel assays and custom cell sorting applications. Collaborative projects encompass a broad scope of research topics and often result from a group effort among several of the Institute’s scientific support teams.

Technology 

Cytometry Analyzers

  • BioRad ZE5 Cell Analyzer
  • Sony EC800 Flow Cytometry Analyzer

High Speed Cell Sorters

  • BD FACSymphony S6 Cell Sorter
  • BD Influx Cell Sorter
  • BD FACSMelody Cell Sorter

Imaging Equipment

  • Amnis ImageStream MkII Imaging Flow Cytometer
  • Nexcelom Celigo Imaging Cytometer

Macroparticle Sorter

  • Union Biometrica CoPas Large Particle Sorter
Team Contact 
Jeffrey S. Haug, BS
Head, Cytometry

After graduating from the University of Missouri, Kansas City, with a BS in biology, Jeffrey S. Haug held several research and clinical positions, in which he relied on cytometric tools to gain new insights into cancer biology. In 1994, he joined the Washington University School of Medicine in the Division of Bone Marrow Transplantation, St. Louis, where he actively participated in both clinical and basic stem cell research. He designed custom flow cytometry applications for the bone marrow transplant division that were the most sensitive, accurate and rapid cytometric assays for blood stem cells available at the time. In 2000, Haug was recruited to the Stowers Institute to establish a Cytometry Facility, which he manages based on the collaborative style of core resource management that he developed at Washington University.

Featured Publications 
Sardiu ME, Box AC, Haug JS, Washburn MP. [Epub ahead of print] Mol Omics. 2020 Sep 14;. doi: 10.1039/d0mo00039f.
Box A, DeLay M, Tighe S, Chittur SV, Bergeron A, Cochran M, Lopez P, Meyer EM, Saluk A, Thornton S, Brundage K. J Biomol Tech: JBT. 2020;31:100-111.
Peuß R, Box AC, Chen S, Wang Y, Tsuchiya D, Persons JL, Kenzior A, Maldonado E, Krishnan J, Scharsack JP, Slaughter BD, Rohner N. Nat Ecol Evol. 2020 Jul 20. doi: 10.1038/s41559-020-1234-2. Online ahead of print. PMID: 32690906
Story B, Ma X, Ishihara K, Li H, Hall K, Peak A, Anoja P, Park J, Haug J, Blanchette M, Xie T. Life Sci Alliance. 2019 Oct;2(5). doi: 10.26508/lsa.201800211.
Lush ME, Diaz DC, Koenecke N, Baek S, Boldt H, St Peter MK, Gaitan-Escudero T, Romero-Carvajal A, Busch-Nentwich EM, Perera AG, Hall KE, Peak A, Haug JS, Piotrowski T. Elife. 2019 Jan 25;8. doi: 10.7554/eLife.44431.
Li Z, Qian P, Shao W, Shi H, He XC, Gogol M, Yu Z, Wang Y, Qi M, Zhu Y, Perry JM, Zhang K, Tao F, Zhou K, Hu D, Han Y, Zhao C, Alexander R, Xu H, Chen S, Peak A, Hall K, Peterson M, Perera A, Haug JS, Parmely T, Li H, Shen B, Zeitlinger J, He C, Li L. Cell Res. Epub 2018 Jul 31. 2018 Sep;28(9):904-917. doi: 10.1038/s41422-018-0072-0.
© 2021 Stowers Institute for Medical Research